immunosorbent assay elisa quantikine elisa human cd163 and scd14 immunoassay  (R&D Systems)

Journal: Journal of Antimicrobial Chemotherapy

Article Title: No accelerated progression of subclinical atherosclerosis with integrase strand transfer inhibitors compared to non-nucleoside reverse transcriptase inhibitors

doi: 10.1093/jac/dkae383

Figure Lengend Snippet: Characteristics of patients according to the antiretroviral regimen composition

Article Snippet: D-dimer [Human D2D (D-Dimer) ELISA Kit], soluble ICAM-1 (sICAM-1) [Human ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA Kit], soluble CD14 (sCD14) [Human sCD14 (Soluble Cluster of Differentiation 14) ELISA Kit] and soluble CD163 (sCD163) [Human sCD163 (Soluble Cluster of Differentiation 163) ELISA Kit] were measured by enzyme-linked immunosorbent assay (ELISA Kits, Elabscience Biotechnology Inc., USA) with an automated instrument (Dynex DS2 ® ELISA system).

Techniques: Cell Counting

Journal: Journal of Antimicrobial Chemotherapy

Article Title: No accelerated progression of subclinical atherosclerosis with integrase strand transfer inhibitors compared to non-nucleoside reverse transcriptase inhibitors

doi: 10.1093/jac/dkae383

Figure Lengend Snippet: Characteristics of patients according to the progression of cIMT during the 2 year follow-up

Article Snippet: D-dimer [Human D2D (D-Dimer) ELISA Kit], soluble ICAM-1 (sICAM-1) [Human ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA Kit], soluble CD14 (sCD14) [Human sCD14 (Soluble Cluster of Differentiation 14) ELISA Kit] and soluble CD163 (sCD163) [Human sCD163 (Soluble Cluster of Differentiation 163) ELISA Kit] were measured by enzyme-linked immunosorbent assay (ELISA Kits, Elabscience Biotechnology Inc., USA) with an automated instrument (Dynex DS2 ® ELISA system).

Techniques: Cell Counting

Journal: Journal of Antimicrobial Chemotherapy

Article Title: No accelerated progression of subclinical atherosclerosis with integrase strand transfer inhibitors compared to non-nucleoside reverse transcriptase inhibitors

doi: 10.1093/jac/dkae383

Figure Lengend Snippet: Univariate and multivariate analysis of factors associated with cIMT increase analysed as a continuous variable during the 2 year follow-up

Article Snippet: D-dimer [Human D2D (D-Dimer) ELISA Kit], soluble ICAM-1 (sICAM-1) [Human ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA Kit], soluble CD14 (sCD14) [Human sCD14 (Soluble Cluster of Differentiation 14) ELISA Kit] and soluble CD163 (sCD163) [Human sCD163 (Soluble Cluster of Differentiation 163) ELISA Kit] were measured by enzyme-linked immunosorbent assay (ELISA Kits, Elabscience Biotechnology Inc., USA) with an automated instrument (Dynex DS2 ® ELISA system).

Techniques: Cell Counting

Journal: Journal of Antimicrobial Chemotherapy

Article Title: No accelerated progression of subclinical atherosclerosis with integrase strand transfer inhibitors compared to non-nucleoside reverse transcriptase inhibitors

doi: 10.1093/jac/dkae383

Figure Lengend Snippet: Patients’ characteristics according to the antiretroviral regimen composition after propensity score matching

Article Snippet: D-dimer [Human D2D (D-Dimer) ELISA Kit], soluble ICAM-1 (sICAM-1) [Human ICAM-1/CD54 (intercellular adhesion molecule 1) ELISA Kit], soluble CD14 (sCD14) [Human sCD14 (Soluble Cluster of Differentiation 14) ELISA Kit] and soluble CD163 (sCD163) [Human sCD163 (Soluble Cluster of Differentiation 163) ELISA Kit] were measured by enzyme-linked immunosorbent assay (ELISA Kits, Elabscience Biotechnology Inc., USA) with an automated instrument (Dynex DS2 ® ELISA system).

Techniques: Cell Counting

Journal: Translational Neuroscience

Article Title: Activating α7nAChR suppresses systemic inflammation by mitigating neuroinflammation of the medullary visceral zone in sepsis in a rat model

doi: 10.1515/tnsci-2022-0345

Figure Lengend Snippet: Concentration of serum cytokines among different groups of rats. TNF-α: tumor necrosis factor alpha; IL-6: interleukin 6; IL-10: interleukin 10; sCD14: soluble CD14, precepsin; HMGB-1: high mobility group box-1. (a–c) Serum concentration (pg/mL) of TNF-α, IL-6, and IL-10 among different groups; (d) serum sCD14 concentration (ng/mL) among different groups, usually as an inflammatory marker in early stage of sepsis; (e) serum concentration of HMGB1 (pg/mL), usually as an inflammatory marker in late stage of sepsis; sepsis causes serum level of inflammatory cytokines, including TNF-α, IL-6, IL-10, sCD14, and HMGB1 significantly increased; these cytokines levels in the model group, the GTS-21 group, and the MLA group were significantly higher than those in the control group and the sham group, except IL-10 and sCD14, GTS-21 significantly decreased the other cytokines’ level; all inflammatory cytokines levels reached the highest in the MLA group, and there were significant differences when compared to the GST-21 groups. * P < 0.05; ** P < 0.01.

Article Snippet: 100 μL of serum were taken to the wells of these kits such as TNF-α (batch number: E-EL-R0019c; Elabscience), IL-6 (batch number: E-EL-R0015c; Elabscience), IL-10 (lot number: E-EL-R0016c; Elabscience), HMGB1 (batch number: E-EL-R0505c; Elabscience), and sCD14 (batch number: CSB-E11178r; Cusabio), respectively, According to the manufacturer’s instructions, after such steps as incubation, washing, reaction with working solution and densitometry, serum contents of TNF-α, IL-6, IL-10, HMGB1, and sCD14 were determined with ELISA.

Techniques: Concentration Assay, Marker, Control

Journal: Neural regeneration research

Article Title: Hemorrhagic transformation in patients with large-artery atherosclerotic stroke is associated with the gut microbiota and lipopolysaccharide.

doi: 10.4103/1673-5374.385846

Figure Lengend Snippet: Figure 6 ｜Key component of LPS-induced inflammation in a rat model of HT. (A) Flowchart of the HT induction procedure. (B). Blood glucose levels throughout surgery in the HT and non-HT groups. (C) Survival curve and number at risk, log-rank test. (D) Representative images of brain slices stained with 2,3,5-triphenyltetrazolium chloride (TTC) stain showing ischemic stroke and hemorrhage transformation, as well as analysis of infarction volume in the different groups. The white arrow indicates infarction, and the red arrow indicates HT. Red areas in the TTC-stained slices indicate no infarction, and white areas in the TTC-stained slices indicate infarction. (E) The hemoglobin content in the ischemic hemisphere, which is used as a measure of bleeding severity. (F) Neurological deficits measured by Garcia score 1, 3, and 5 days after MCAO surgery. (G and H) Sensorimotor function in the HT and non-HT groups. Graphs show contact time (G) and time to removal of the sticker (H) from the contralateral forepaw 1 day before and 2 and 4 days after MCAO (n = 6 rats per group). (I–K). Plasma levels of LPS, LBP, and sCD14 were measured in the different groups. Data are presented as mean ± SD. n = 6–14 per group. *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test, one-way analysis of variance followed by post-hoc Tukey’s multiple comparison test or the Kruskal-Wallis H test was used as appropriate). Average values from three technical replicates of each biological replicate are shown. HT: Hemorrhagic transformation; LBP: LPS-binding protein; LPS: lipopolysaccharide; MCAO: middle cerebral artery occlusion; ns: not significant; sCD14: solube CD14.

Article Snippet: Enzyme-linked immunosorbent assay was used for measuring human LBP (Cusabio Science Co., Ltd., Wuhan, China), human sCD14 (Cusabio Science Co., Ltd.), human zonulin (Cusabio Science Co., Ltd.), human matrix metalloproteinase 9 (MMP9) (Cusabio Science Co., Ltd.), mouse LBP (Elabscience, Wuhan, China), and mouse sCD14 (Elabscience) concentrations.

Techniques: Staining, Transformation Assay, Clinical Proteomics, Comparison, Binding Assay

Journal: Neural regeneration research

Article Title: Hemorrhagic transformation in patients with large-artery atherosclerotic stroke is associated with the gut microbiota and lipopolysaccharide.

doi: 10.4103/1673-5374.385846

Figure Lengend Snippet: Figure 5 ｜Gut microbial gene pathways are altered in HT subjects. (A, B) Spearman analysis of LPS-related inflammation and barrier function markers correlated with bacteria (A) and alpha diversity (B). (C) PICRUSt analysis based on the KEGG database was used to predict microbial metabolic function and analyze functional differences (as detected by STAMP analysis). (D, E) Pathways relevant to LPS and LBP biosynthesis. Data obtained from the PICRUSt server. n = 15–17 per group. One-way analysis of variance followed by post-hoc Tukey’s multiple comparison test or the Kruskal- Wallis H test was used. HC: Healthy controls; HT: stroke patients with hemorrhagic transformation; KEGG: Kyoto Encyclopedia of Genes and Genomes; LBP: LPS-binding protein; LPS: lipopolysaccharide; MMP9: matrix metalloproteinase 9; non-HT: stroke patients without hemorrhagic transformation; PICRUSt: phylogenetic Investigation of Communities by Reconstruction of Unobserved States; sCD14: solube CD14.

Article Snippet: Enzyme-linked immunosorbent assay was used for measuring human LBP (Cusabio Science Co., Ltd., Wuhan, China), human sCD14 (Cusabio Science Co., Ltd.), human zonulin (Cusabio Science Co., Ltd.), human matrix metalloproteinase 9 (MMP9) (Cusabio Science Co., Ltd.), mouse LBP (Elabscience, Wuhan, China), and mouse sCD14 (Elabscience) concentrations.

Techniques: Bacteria, Functional Assay, Comparison, Transformation Assay, Binding Assay

Journal: Neural regeneration research

Article Title: Hemorrhagic transformation in patients with large-artery atherosclerotic stroke is associated with the gut microbiota and lipopolysaccharide.

doi: 10.4103/1673-5374.385846

Figure Lengend Snippet: Figure 7 ｜ Changes in LPS-related signaling in recipient rats after FMT. (A) Fecal microbiota transplant experimental design. Rats were treated with antibiotics for 2 weeks and then received fresh stool from either HT or non-HT donors by oral gavage. After 2 weeks of daily gavage with donor stool, MCAO was induced in the recipient rats. Five days later they were killed to quantify infarct volume and hemorrhage transformation. (B) Blood glucose levels in the different groups. (C) Infarct volumes in the different groups. The white arrow indicates infarction, and the red arrow indicates HT. (D) Survival curve and number at risk. (E) Calculation of hemorrhagic score according to a four-point rubric. (F) Brain hemoglobin levels were evaluated 5 d after MCAO. (G–I) Neurologic function as evaluated by Garcia score and sticker removal test. (J–L) LPS, LBP, and sCD14 concentrations in the different groups. Average values of three technical replicates performed for each biological replicate are shown. Data are presented as mean ± SD. n = 8–11 per group. *P < 0.05, **P < 0.01, ***P < 0.001. Statistical significance was determined by Student’s t-test or Mann-Whitney U test. FMT: Fecal microbiota transplantation; HT: hemorrhagic transformation; LBP: LPS-binding protein; LPS: lipopolysaccharide; MCAO: middle cerebral artery occlusion; sCD14: solube CD14.

Article Snippet: Enzyme-linked immunosorbent assay was used for measuring human LBP (Cusabio Science Co., Ltd., Wuhan, China), human sCD14 (Cusabio Science Co., Ltd.), human zonulin (Cusabio Science Co., Ltd.), human matrix metalloproteinase 9 (MMP9) (Cusabio Science Co., Ltd.), mouse LBP (Elabscience, Wuhan, China), and mouse sCD14 (Elabscience) concentrations.

Techniques: Transformation Assay, MANN-WHITNEY, Transplantation Assay, Binding Assay